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	Provedor de dados Electron. J. Biotechnol. (2) Biological Sciences (1) Autor Busse,Nadine (1) Colognesi, Geyci de Oliveira (1) Czermak,Peter (1) Lai,Daowan (1) Liu,Yang (1) Luo,Haiyu (1) Morioka, Luiz Rodrigo Ito (1) Oppermann,Tobias (1) Schwarz,Stefan (1) Suguimoto, Hélio Hiroshi (1) Wang,Jian (1) Xie,Rushan (1) Xu,Dan (1) Xuejiao,Dong (1) Zhang,Xuping (1) Zhou,Ligang (1) Mais... Palavra-chave Microbial biotechnology (3) ATP assay (1) Bacterial pathogen (1) Beta-galactosidase (1) Bioactivities (1) Bovine respiratory disease (1) Cattle industry (1) Central composite design (1) Ciência dos alimentos permeabilizing agent (1) Combination effects (1) Critical parameters (1) Lipopolysaccharides (1) Mannheimia haemolytica (1) Optimal conditions Biotecnologia (1) Polymyxin B (1) Process biotechnology (1) Production (1) Quantification (1) Response surface methodology (1) Virulence (1) Mais... Tipo do documento Journal article (2) Info:eu-repo/semantics/article (1) Ano 2016 (3) País Chile (2) Brazil (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A fast and simple assay to quantify bacterial leukotoxin activity Electron. J. Biotechnol. Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter. Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be... Tipo: Journal article Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006 Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions Electron. J. Biotechnol. Luo,Haiyu; Xu,Dan; Xie,Rushan; Zhang,Xuping; Wang,Jian; Xuejiao,Dong; Lai,Daowan; Zhou,Ligang; Liu,Yang. Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e.,Mn2+,Na+, Mg2+,Zn2+,Cu2+,Fe2+,Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated. Results: Three most effective metal ions (Zn2+,Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+,Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+,Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing... Tipo: Journal article Palavras-chave: Combination effects; Production; Microbial biotechnology; Process biotechnology; Bioactivities; Central composite design; Response surface methodology. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600002 Permeabilization of Saccharomyces fragilis IZ 275 cells with ethanol to obtain a biocatalyst with lactose hydrolysis capacity Biological Sciences Morioka, Luiz Rodrigo Ito; Colognesi, Geyci de Oliveira; Suguimoto, Hélio Hiroshi. The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. The Saccharomyces fragilis IZ 275 yeast cells were permeabilized with ethanol, as permeabilizing agent. To optimize the permeabilization conditions were used the design of Box-Behnken 15 trials (3 central points). The independent variables and their levels were ethanol (29, 32 and 35%), temperature (15, 20 and 25°C) and time (15, 20 and 25 min). The answer (Y) function has beta-galactosidase activity (U mg-1). The optimum conditions for obtaining a high enzymatic activity were observed in 35% ethanol concentration, temperature 15ºC and 20 min. treatment time. The maximum activity of the enzyme beta-galactosidase obtained was 10.59 U mg-1. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciência dos alimentos permeabilizing agent; Beta-galactosidase; Microbial biotechnology; Optimal conditions Biotecnologia. Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/29220 Registros recuperados: 3 Primeira ... 1 ... Última

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